growth medium mv2 supplement pack Search Results


endothelial-cell growth medium mv2  (Lonza)
90
Lonza endothelial-cell growth medium mv2
Endothelial Cell Growth Medium Mv2, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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endothelial cell growth medium mv2 kit  (PromoCell)
95
PromoCell endothelial cell growth medium mv2 kit

Endothelial Cell Growth Medium Mv2 Kit, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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endothelial cell growth medium mv2  (Innoprot Inc)
95
Innoprot Inc endothelial cell growth medium mv2

Endothelial Cell Growth Medium Mv2, supplied by Innoprot Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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egm-mv2 bullet kit  (Lonza)
90
Lonza egm-mv2 bullet kit

Egm Mv2 Bullet Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mv2 growth medium with tnfα  (R&D Systems)
99
R&D Systems mv2 growth medium with tnfα
(A) Effect of COMP-Ang1 on F-actin remodeling and VE-Cadherin distribution in isolated human pulmonary microvascular endothelial cell. HPMECs grown on glass coverslips were transfected for 24h with control mRNA LUC or with mRNA-76 encoding COMP-Ang1 with or without temporal addition of 10 ng/ml <t>TNFα,</t> followed by immunofluorescence staining with VE-Cadherin-specific antibody and with Alexa Fluor 594 phalloidin to detect actin filaments. Arrows indicate disrupted VE-Cadherin complexes. B) Transcellular electrical resistance (TER) measurements of HPMEC monolayers. HPMEC were incubated for 30 min (top) or 60 min (bottom) with supernatant from mRNA-76 or with mRNA LUC (Luciferase) and the treated with supernatants from Hela cells transfected for 24 h with control mRNA LUC or mRNA-76 encoding COMP-Ang1 and stimulated with indicated amount of pneumolysin (PLY; 0.25, 0.5, 0.75, 1.0 μg/ml). PLY stimulation decreased TER of HPMEC monolayers, displaying loss of endothelial integrity when incubated with control Luciferase supernatant (solid curve). Preincubation with COMP-Ang1 containing supernatant attenuated the PLY-induced TER decrease (purple lines dashed versus solid).
Mv2 Growth Medium With Tnfα, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spherical ichells consisting of mv2+ and [pw12o40] 3  (Verlag GmbH)
90
Verlag GmbH spherical ichells consisting of mv2+ and [pw12o40] 3
(A) Effect of COMP-Ang1 on F-actin remodeling and VE-Cadherin distribution in isolated human pulmonary microvascular endothelial cell. HPMECs grown on glass coverslips were transfected for 24h with control mRNA LUC or with mRNA-76 encoding COMP-Ang1 with or without temporal addition of 10 ng/ml <t>TNFα,</t> followed by immunofluorescence staining with VE-Cadherin-specific antibody and with Alexa Fluor 594 phalloidin to detect actin filaments. Arrows indicate disrupted VE-Cadherin complexes. B) Transcellular electrical resistance (TER) measurements of HPMEC monolayers. HPMEC were incubated for 30 min (top) or 60 min (bottom) with supernatant from mRNA-76 or with mRNA LUC (Luciferase) and the treated with supernatants from Hela cells transfected for 24 h with control mRNA LUC or mRNA-76 encoding COMP-Ang1 and stimulated with indicated amount of pneumolysin (PLY; 0.25, 0.5, 0.75, 1.0 μg/ml). PLY stimulation decreased TER of HPMEC monolayers, displaying loss of endothelial integrity when incubated with control Luciferase supernatant (solid curve). Preincubation with COMP-Ang1 containing supernatant attenuated the PLY-induced TER decrease (purple lines dashed versus solid).
Spherical Ichells Consisting Of Mv2+ And [Pw12o40] 3, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mv2+ infiltrated within a kagome-style hollow-core photonic crystal fiber (hc-pcf)  (KAGOME CO)
90
KAGOME CO mv2+ infiltrated within a kagome-style hollow-core photonic crystal fiber (hc-pcf)
(A) Effect of COMP-Ang1 on F-actin remodeling and VE-Cadherin distribution in isolated human pulmonary microvascular endothelial cell. HPMECs grown on glass coverslips were transfected for 24h with control mRNA LUC or with mRNA-76 encoding COMP-Ang1 with or without temporal addition of 10 ng/ml <t>TNFα,</t> followed by immunofluorescence staining with VE-Cadherin-specific antibody and with Alexa Fluor 594 phalloidin to detect actin filaments. Arrows indicate disrupted VE-Cadherin complexes. B) Transcellular electrical resistance (TER) measurements of HPMEC monolayers. HPMEC were incubated for 30 min (top) or 60 min (bottom) with supernatant from mRNA-76 or with mRNA LUC (Luciferase) and the treated with supernatants from Hela cells transfected for 24 h with control mRNA LUC or mRNA-76 encoding COMP-Ang1 and stimulated with indicated amount of pneumolysin (PLY; 0.25, 0.5, 0.75, 1.0 μg/ml). PLY stimulation decreased TER of HPMEC monolayers, displaying loss of endothelial integrity when incubated with control Luciferase supernatant (solid curve). Preincubation with COMP-Ang1 containing supernatant attenuated the PLY-induced TER decrease (purple lines dashed versus solid).
Mv2+ Infiltrated Within A Kagome Style Hollow Core Photonic Crystal Fiber (Hc Pcf), supplied by KAGOME CO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human lung microvascular endothelial cells (hmvecs)  (Lonza)
90
Lonza human lung microvascular endothelial cells (hmvecs)
(A) Effect of COMP-Ang1 on F-actin remodeling and VE-Cadherin distribution in isolated human pulmonary microvascular endothelial cell. HPMECs grown on glass coverslips were transfected for 24h with control mRNA LUC or with mRNA-76 encoding COMP-Ang1 with or without temporal addition of 10 ng/ml <t>TNFα,</t> followed by immunofluorescence staining with VE-Cadherin-specific antibody and with Alexa Fluor 594 phalloidin to detect actin filaments. Arrows indicate disrupted VE-Cadherin complexes. B) Transcellular electrical resistance (TER) measurements of HPMEC monolayers. HPMEC were incubated for 30 min (top) or 60 min (bottom) with supernatant from mRNA-76 or with mRNA LUC (Luciferase) and the treated with supernatants from Hela cells transfected for 24 h with control mRNA LUC or mRNA-76 encoding COMP-Ang1 and stimulated with indicated amount of pneumolysin (PLY; 0.25, 0.5, 0.75, 1.0 μg/ml). PLY stimulation decreased TER of HPMEC monolayers, displaying loss of endothelial integrity when incubated with control Luciferase supernatant (solid curve). Preincubation with COMP-Ang1 containing supernatant attenuated the PLY-induced TER decrease (purple lines dashed versus solid).
Human Lung Microvascular Endothelial Cells (Hmvecs), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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simport mv2 game  (Simport Scientific)
90
Simport Scientific simport mv2 game
(A) Effect of COMP-Ang1 on F-actin remodeling and VE-Cadherin distribution in isolated human pulmonary microvascular endothelial cell. HPMECs grown on glass coverslips were transfected for 24h with control mRNA LUC or with mRNA-76 encoding COMP-Ang1 with or without temporal addition of 10 ng/ml <t>TNFα,</t> followed by immunofluorescence staining with VE-Cadherin-specific antibody and with Alexa Fluor 594 phalloidin to detect actin filaments. Arrows indicate disrupted VE-Cadherin complexes. B) Transcellular electrical resistance (TER) measurements of HPMEC monolayers. HPMEC were incubated for 30 min (top) or 60 min (bottom) with supernatant from mRNA-76 or with mRNA LUC (Luciferase) and the treated with supernatants from Hela cells transfected for 24 h with control mRNA LUC or mRNA-76 encoding COMP-Ang1 and stimulated with indicated amount of pneumolysin (PLY; 0.25, 0.5, 0.75, 1.0 μg/ml). PLY stimulation decreased TER of HPMEC monolayers, displaying loss of endothelial integrity when incubated with control Luciferase supernatant (solid curve). Preincubation with COMP-Ang1 containing supernatant attenuated the PLY-induced TER decrease (purple lines dashed versus solid).
Simport Mv2 Game, supplied by Simport Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ebm-mv2  (Lonza)
90
Lonza ebm-mv2
(A) Effect of COMP-Ang1 on F-actin remodeling and VE-Cadherin distribution in isolated human pulmonary microvascular endothelial cell. HPMECs grown on glass coverslips were transfected for 24h with control mRNA LUC or with mRNA-76 encoding COMP-Ang1 with or without temporal addition of 10 ng/ml <t>TNFα,</t> followed by immunofluorescence staining with VE-Cadherin-specific antibody and with Alexa Fluor 594 phalloidin to detect actin filaments. Arrows indicate disrupted VE-Cadherin complexes. B) Transcellular electrical resistance (TER) measurements of HPMEC monolayers. HPMEC were incubated for 30 min (top) or 60 min (bottom) with supernatant from mRNA-76 or with mRNA LUC (Luciferase) and the treated with supernatants from Hela cells transfected for 24 h with control mRNA LUC or mRNA-76 encoding COMP-Ang1 and stimulated with indicated amount of pneumolysin (PLY; 0.25, 0.5, 0.75, 1.0 μg/ml). PLY stimulation decreased TER of HPMEC monolayers, displaying loss of endothelial integrity when incubated with control Luciferase supernatant (solid curve). Preincubation with COMP-Ang1 containing supernatant attenuated the PLY-induced TER decrease (purple lines dashed versus solid).
Ebm Mv2, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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endothelial growth mv2 media  (Lonza)
90
Lonza endothelial growth mv2 media
(A) Effect of COMP-Ang1 on F-actin remodeling and VE-Cadherin distribution in isolated human pulmonary microvascular endothelial cell. HPMECs grown on glass coverslips were transfected for 24h with control mRNA LUC or with mRNA-76 encoding COMP-Ang1 with or without temporal addition of 10 ng/ml <t>TNFα,</t> followed by immunofluorescence staining with VE-Cadherin-specific antibody and with Alexa Fluor 594 phalloidin to detect actin filaments. Arrows indicate disrupted VE-Cadherin complexes. B) Transcellular electrical resistance (TER) measurements of HPMEC monolayers. HPMEC were incubated for 30 min (top) or 60 min (bottom) with supernatant from mRNA-76 or with mRNA LUC (Luciferase) and the treated with supernatants from Hela cells transfected for 24 h with control mRNA LUC or mRNA-76 encoding COMP-Ang1 and stimulated with indicated amount of pneumolysin (PLY; 0.25, 0.5, 0.75, 1.0 μg/ml). PLY stimulation decreased TER of HPMEC monolayers, displaying loss of endothelial integrity when incubated with control Luciferase supernatant (solid curve). Preincubation with COMP-Ang1 containing supernatant attenuated the PLY-induced TER decrease (purple lines dashed versus solid).
Endothelial Growth Mv2 Media, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mv2 bullet kit  (Lonza)
90
Lonza mv2 bullet kit
(A) Effect of COMP-Ang1 on F-actin remodeling and VE-Cadherin distribution in isolated human pulmonary microvascular endothelial cell. HPMECs grown on glass coverslips were transfected for 24h with control mRNA LUC or with mRNA-76 encoding COMP-Ang1 with or without temporal addition of 10 ng/ml <t>TNFα,</t> followed by immunofluorescence staining with VE-Cadherin-specific antibody and with Alexa Fluor 594 phalloidin to detect actin filaments. Arrows indicate disrupted VE-Cadherin complexes. B) Transcellular electrical resistance (TER) measurements of HPMEC monolayers. HPMEC were incubated for 30 min (top) or 60 min (bottom) with supernatant from mRNA-76 or with mRNA LUC (Luciferase) and the treated with supernatants from Hela cells transfected for 24 h with control mRNA LUC or mRNA-76 encoding COMP-Ang1 and stimulated with indicated amount of pneumolysin (PLY; 0.25, 0.5, 0.75, 1.0 μg/ml). PLY stimulation decreased TER of HPMEC monolayers, displaying loss of endothelial integrity when incubated with control Luciferase supernatant (solid curve). Preincubation with COMP-Ang1 containing supernatant attenuated the PLY-induced TER decrease (purple lines dashed versus solid).
Mv2 Bullet Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: iScience

Article Title: Negative regulation of lymphangiogenesis by Tenascin-C delays the resolution of inflammation

doi: 10.1016/j.isci.2025.111756

Figure Lengend Snippet:

Article Snippet: Endothelial Cell Growth Medium MV2 kit , Promo Cell , C22121.

Techniques: Recombinant, Membrane, Blocking Assay, Western Blot, Ointment, Proliferation Assay, Software

(A) Effect of COMP-Ang1 on F-actin remodeling and VE-Cadherin distribution in isolated human pulmonary microvascular endothelial cell. HPMECs grown on glass coverslips were transfected for 24h with control mRNA LUC or with mRNA-76 encoding COMP-Ang1 with or without temporal addition of 10 ng/ml TNFα, followed by immunofluorescence staining with VE-Cadherin-specific antibody and with Alexa Fluor 594 phalloidin to detect actin filaments. Arrows indicate disrupted VE-Cadherin complexes. B) Transcellular electrical resistance (TER) measurements of HPMEC monolayers. HPMEC were incubated for 30 min (top) or 60 min (bottom) with supernatant from mRNA-76 or with mRNA LUC (Luciferase) and the treated with supernatants from Hela cells transfected for 24 h with control mRNA LUC or mRNA-76 encoding COMP-Ang1 and stimulated with indicated amount of pneumolysin (PLY; 0.25, 0.5, 0.75, 1.0 μg/ml). PLY stimulation decreased TER of HPMEC monolayers, displaying loss of endothelial integrity when incubated with control Luciferase supernatant (solid curve). Preincubation with COMP-Ang1 containing supernatant attenuated the PLY-induced TER decrease (purple lines dashed versus solid).

Journal: bioRxiv

Article Title: Spatial expression of an mRNA encoding Tie2-agonist in the capillary endothelium of the lung prevents pulmonary vascular leakage

doi: 10.1101/2022.10.12.511878

Figure Lengend Snippet: (A) Effect of COMP-Ang1 on F-actin remodeling and VE-Cadherin distribution in isolated human pulmonary microvascular endothelial cell. HPMECs grown on glass coverslips were transfected for 24h with control mRNA LUC or with mRNA-76 encoding COMP-Ang1 with or without temporal addition of 10 ng/ml TNFα, followed by immunofluorescence staining with VE-Cadherin-specific antibody and with Alexa Fluor 594 phalloidin to detect actin filaments. Arrows indicate disrupted VE-Cadherin complexes. B) Transcellular electrical resistance (TER) measurements of HPMEC monolayers. HPMEC were incubated for 30 min (top) or 60 min (bottom) with supernatant from mRNA-76 or with mRNA LUC (Luciferase) and the treated with supernatants from Hela cells transfected for 24 h with control mRNA LUC or mRNA-76 encoding COMP-Ang1 and stimulated with indicated amount of pneumolysin (PLY; 0.25, 0.5, 0.75, 1.0 μg/ml). PLY stimulation decreased TER of HPMEC monolayers, displaying loss of endothelial integrity when incubated with control Luciferase supernatant (solid curve). Preincubation with COMP-Ang1 containing supernatant attenuated the PLY-induced TER decrease (purple lines dashed versus solid).

Article Snippet: Media containing formulations was removed and replaced with 10 % MV2 growth medium for 16 h. For TNFα treatment, 10 % MV2 growth medium with TNFα (R&D Systems, cat. #210-TA) or vehicle was spiked into each well for a final concentration of 10 ng/ml for a further 6 h. Cells were fixed in 4 % PFA (Thermo Scientific, cat. #15670799) for 10 min at room temperature (RT).

Techniques: Isolation, Transfection, Control, Immunofluorescence, Staining, Incubation, Luciferase